A novel antimicrobial peptide from the loach, Misgurnus anguillicaudatus

FEBS Letters

Kim, Mi Sun²  Park, In Yup¹  Kim, Sun Chang¹  Park, Chan Bae¹  Lee, Jae Hyun¹ 

[1] Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Yusong-gu Kusong-dong, Taejeon 305-701, Korea;Korea Institute of Energy Research, Biomass Team, Taejeon 305-343, South Korea

关键词: Antimicrobial;    Peptide;    Mudfish;    Loach;    (Misgurnus anguillicaudatus);   

DOI  :  10.1016/S0014-5793(97)00684-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chromatography. The complete amino acid sequence of misgurin, which was determined by an automated amino acid sequencer, was Arg–Gln–Arg–Val–Glu–Glu–Leu–Ser–Lys–Phe–Ser–Lys–Lys–Gly–Ala–Ala–Ala–Arg–Arg–Arg–Lys. Misgurin is a strongly basic peptide which has 5 arginine and 4 lysine residues. Comparison of the amino acid sequence with those of other known antimicrobial peptides revealed that misgurin was a novel antimicrobial peptide. Misgurin showed a strong antimicrobial activity in vitro against a broad spectrum of microorganisms without significant hemolytic activity and was about 6 times more potent than magainin 2. Scanning electron microscopy confirmed that the peptide caused damage to the cell membrane by a pore-forming mechanism similar to that of magainin 2. This damage occurred at the minimal inhibition concentration (MIC), but at higher concentration than MIC it lysed the cell.

【 授权许可】

Unknown