Prophenin‐1, an exceptionally proline‐rich antimicrobial peptide from porcine leukocytes

FEBS Letters

Aleshina, Galina M³  Kokryakov, Vladimir N³  Swiderek, Kristine M²  Harwig, Sylvia S.L¹  Zhao, Chengquan¹  Lehrer, Robert I¹ 

[1] Department of Medicine, UCLA School of Medicine, Los Angeles CA 90095, USA;Division of Immunology, Beckman Research Institute, City of Hope, Duarte CA 91010, USA;Department of General Pathology and Pathophysiology, Institute for Experimental Medicine, 19736 St. Petersburg, Russian Federation

关键词: Antimicrobial;    Cathelin;    Peptide;    Porcine leukocyte;    Proline-rich;   

DOI  :  10.1016/0014-5793(95)00210-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We purified and characterized an unusual antimicrobial peptide, prophenin-1 (PF-1), from porcine leukocytes. The peptide had a mass of 8,683 and contained 79 residues, including 42 (53.2%) prolines and 15 (19.0%) phenylalanines. Its N-terminal 60 residues consisted of three perfect and three nearly perfect repeats of a decamer, FPPPNFPGPR. Prophenin-1 was encoded on a cathelin-containing precursor and showed substantially more activity against E. coli, a Gram-negative bacterium, than against Listeria monocytogenes, a Gram-positive organism, in vitro.

【 授权许可】

Unknown