Hevamine, a chitinase from the rubber tree Hevea brasiliensis, cleaves peptidoglycan between the C‐1 of N‐acetylglucosamine and C‐4 of N‐acetylmuramic acid and therefore is not a lysozyme

FEBS Letters

van Koningsveld, Gerrit A.²  Jeronimus-Stratingh, Margot¹  Bokma, Evert²  Beintema, Jaap J.² 

[1] Centrum voor Farmacie, Rijksuniversiteit Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands;Biochemisch Laboratorium, Rijksuniversiteit Groningen, Nijenborgh 4. 9747 AG Groningen, The Netherlands

关键词: Lysozyme;    Chitinase;    Cleavage specificity;    Peptidoglycan;    NAG;    N-acetylglucosamine;    NAM;    N-acetylmuramate;    p-ABEE;    ethyl-p-aminobenzoate;   

DOI  :  10.1016/S0014-5793(97)00682-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hevamine is a chitinase from the rubber tree Hevea brasiliensis and belongs to the family 18 glycosyl hydrolases. In this paper the cleavage specificity of hevamine for peptidoglycan was studied by HPLC and mass-spectrometry analysis of enzymatic digests. The results clearly showed that the enzyme cleaves between the C-1 of a N-acetylglucosamine and the C-4 of a N-acetylmuramate residue. This means that hevamine, and very likely also other family 18 glycosyl hydrolases which cleave peptidoglycan, cannot be classified as lysozymes.

【 授权许可】

Unknown