FEBS Letters | |
Estrogen receptor mRNA in mineralized tissues of rainbow trout: calcium mobilization by estrogen | |
Valotaire, Yves1  Russell, R.Graham G.3  Pakdel, Farzad1  Armour, Kenneth J.2  Henderson, Ian W.2  Lehane, David B.2  | |
[1] Laboratoire de Biologie Moléculaire, UPRESA CNRS No. 256, Université de Rennes I, 35042 Rennes Cedex, France;Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK;Department of Human Metabolism and Clinical Biochemistry, University Medical School, Sheffield S10 2RX, UK | |
关键词: 17β-Estradiol; Estrogen receptor; Teleost; Bone; Scale; | |
DOI : 10.1016/S0014-5793(97)00680-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020304574ZK.pdf | 474KB | download |