ERβ: Identification and characterization of a novel human estrogen receptor

FEBS Letters

Mosselman, Sietse¹  Dijkema, Rein¹  Polman, Jan¹ 

[1] N.V. Organon, Department of Biotechnology and Biochemistry, P.O. Box 20, 5340 BH Oss, The Netherlands

关键词: Estrogen receptor;    17β-Estradiol;    Anti-estrogens;    Transactivation;    Human;   

DOI  :  10.1016/0014-5793(96)00782-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel estrogen receptor (hereinafter referred to as ERβ) was cloned using degenerate PCR primers. A comparison of the amino acid sequence of ERβ with the ‘classical’ ER (ERα) shows a high degree of conservation of the DNA-binding domain (96%), and of the ligand-binding domain (58%). In contrast, the A/B domain, the hinge region and the F-domain are not conserved. Northern blot analysis revealed that ERβ is expressed in human thymus, spleen, ovary and testis. Transient transfections of an ERβ expression construct together with an ERE-based reporter construct in CHO cells clearly demonstrated transactivation of ERβ by 17β-estradiol. In addition, the ERα antagonist ICI-164384 is a potent antagonist for ERβ as well. Interestingly, the level of transactivation by 17β-estradiol is higher for ERα than for ERβ, which may reflect suboptimal conditions for ERβ at the level of the ligand, responsive element or cellular context.

【 授权许可】

Unknown