Ceruloplasmin, transferrin and apotransferrin facilitate iron release from human liver cells

FEBS Letters

Fahmy, Magdy¹  Young, Stephen P¹  Golding, Simon¹ 

[1] Department of Rheumatology, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

关键词: Iron metabolism;    Transferrin;    Ceruloplasmin;    Ferroxidase;    HepG2 cell;   

DOI  :  10.1016/S0014-5793(97)00478-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rate of iron release from HepG2 liver cells was increased not only by extracellular apotransferrin, but also by diferric transferrin, in a non-additive, concentration-dependent manner and to a similar magnitude. This suggests that rapid equilibration between receptor-mediated uptake and the release process determines net iron retention by the liver. Release was also accelerated by ceruloplasmin; most importantly, the effect of this protein was greatest when iron release was occurring rapidly, stimulated by apotransferrin, or under conditions of limited oxygen. Thus iron release involves both apotransferrin and ferrotransferrin, with ceruloplasmin playing a role in tissues with limited oxygen supply, as in the liver in vivo.

【 授权许可】

Unknown