β‐Adrenergic stimulation of interleukin‐1α and interleukin‐6 expression in mouse brown adipocytes

FEBS Letters

Burýšek, Ladislav¹  Houštěk, Josef¹ 

[1] Institute of Physiology, Academy of Sciences of the Czech Republic, Vı́deòňská 1083, 142 20 Prague, Czech Republic

关键词: Brown adipocytes;    Gene expression;    Interleukin-1α;    Interleukin-6;    Adrenergic regulation;    BAT;    brown adipose tissue;    IL-1α;    interleukin-1α;    IL-6;    interleukin-6;    NE;    norepinephrine;    db-cAMP;    dibutyryl-cAMP;   

DOI  :  10.1016/S0014-5793(97)00671-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse brown adipocytes in primary culture were shown to contain high levels of mRNA for interleukin-1α (IL-1α) which could be further stimulated up to 9-fold by norepinephrine (NE). Even higher stimulation by NE, up to 40-fold, was found in case of interleukin-6 (IL-6). Time-course of activation of both genes was biphasic, but the response of IL-6 gene was slower than of IL-1α gene. IL-1α mRNA level reached the maximum after 1 h and the second, lower increase, occurred after 8 h. IL-6 mRNA level showed first maximum after 2 h, but the highest level was found after 8 h. Similarly to NE, the expression of IL-1α and IL-6 genes was stimulated by selective β-adrenergic agonist isoproterenol, β₃-selective agonist CGP-12117, forskoline and db-cAMP. The activation of both genes by CGP-12177 was dose-dependent with the optimum at 100 nM concentration. Stimulation of α-adrenergic receptors by cirazoline and oxymetazoline was without any effect. When the expression of IL-6 was studied at the protein level, the stimulation of IL-6 gene via β₃-receptors resulted in secretion of IL-6 up to the concentration 10 ng/ml culture media in 24 h. The results indicate a new type of regulation of expression of IL-1α and IL-6 genes in brown adipocytes by catecholamines acting via β₃-adrenergic receptors. The resulting increase in IL-6 production by brown adipocytes could significantly contribute to systemic levels of IL-6.

【 授权许可】

Unknown