FEBS Letters | |
Crystal structure of Escherichia coli inorganic pyrophosphatase complexed with SO4 2− | |
Dauter, Zbygnew3  Sklyankina, Vera1  Huber, Robert5  Oganessyan, Vaheh2  Kurilova, Svetlana4  Vorobyeva, Natalya1  Rodina, Elena4  Harutyunyan, Emil2  Mather, Timothy5  Nazarova, Tatjana4  Grigorjeva, Olga1  Avaeva, Svetlana4  Wilson, Keith3  | |
[1] Chemistry Department, Moscow State University, Moscow, Russia;Institute of Crystallography, Russian Academy of Sciences, Moscow, Russia;EMBL Outstation c/o DESY, Hamburg, Germany;A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Vorobyevy Gory, MSU, Moscow 119899, Russia;Institute of Biochemistry, Munich, Germany | |
关键词: Inorganic pyrophosphatase; Escherichia coli; Crystal structure; Complex with sulfate; Cooperativity; | |
DOI : 10.1016/S0014-5793(97)00650-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The three-dimensional structure of inorganic pyrophosphatase from Escherichia coli complexed with sulfate was determined at 2.2 Å resolution using Patterson's search technique and refined to an R-factor of 19.2%. Sulfate may be regarded as a structural analog of phosphate, the product of the enzyme reaction, and as a structural analog of methyl phosphate, the irreversible inhibitor. Sulfate binds to the pyrophosphatase active site cavity as does phosphate and this diminishes molecular symmetry, converting the homohexamer structure form (α3)2 into α3′α3″. The asymmetry of the molecule is manifested in displacements of protein functional groups and some parts of the polypeptide chain and reflects the interaction of subunits and their cooperation. The significance of re-arrangements for pyrophosphatase function is discussed.
【 授权许可】
Unknown
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