Transfer of CD26/dipeptidyl peptidase IV (E.C. 3.5.4.4) from prostasomes to sperm

FEBS Letters

Polci, Andrea¹  Arienti, Giuseppe¹  Palmerini, Carlo A²  Carlini, Enrico¹ 

[1] Istituto di Biochimica e Chimica Medica, Via del Giochetto, 06127 Perugia, Italy;Dipartimento di Biologia Cellulare e Molecolare, Via del Giochetto, 06127 Perugia, Italy

关键词: CD26;    Membrane fusion;    Prostasome;    Dipeptidyl peptidase;    Sperm;    DMSO;    dimethyl sulfoxide;    Gly–Pro–4-Me–2-NA;    glycyl–proline–4-methoxy–2-naphtylamine;    HEPES;    N-[2-hydroxyethylpiperazine]-N′-[2-ethanesulfonic acid];    Mes;    2-[morpholino]ethanesulfonic acid;    4-Me–2-NA;    4-methoxy–2-naphtylamine;    PBS;    phosphate-buffered saline (1.2 mmol·l−1 KH2PO4;    8.1 mmol·l−1 Na2HPO4;    2.7 mmol·l−1 KCl;    138 mmol·l−1 NaCl;    pH 7.2);   

DOI  :  10.1016/S0014-5793(97)00655-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostasomes are vesicles present in human semen. They are secreted by the prostate and contain large amounts of cholesterol and sphingomyelin. Some of their proteins are enzymes. Prostasomes are involved in a number of biological functions. In previous papers we demonstrated that lipid can be transferred from prostasomes to sperm by a fusion process occurring at neutral or slightly acidic pH. In this paper we demonstrate that CD26/dipeptidyl peptidase IV, an enzymatic activity absent in sperm, is transferred to sperm from prostasomes. This may be of particular interest since, by this procedure, sperm may acquire new membrane-bound enzymes and modify the catalytic activity of their surface.

【 授权许可】

Unknown