A new model for the membrane topology of glucose‐6‐phosphatase: the enzyme involved in von Gierke disease

FEBS Letters

Wever, Ron¹  Hemrika, Wieger¹ 

[1] E.C. Slater Institute, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands

关键词: Glucose-6-phosphatase;    Vanadate-containing chloroperoxidase;    Membrane topology;    Glycogen storage disease;   

DOI  :  10.1016/S0014-5793(97)00530-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Very recently we have proposed [Hemrika et al. (1997) Proc. Natl. Acad. Sci. USA 94, 2145–2149] that the active site of the vanadate-containing chloroperoxidase from the fungus Curvularia inaequalis, of which the tertiary structure is known, is structurally very similar to that of the membrane-bound mammalian glucose-6-phosphatases for which no structural data are available. The proposed active site of glucose-6-phosphatase, however, is incompatible with the six transmembrane–helix topology model that is currently used. Here we present a new topology model for glucose-6-phosphatase which is in agreement with all available data.

【 授权许可】

Unknown