Singlet oxygen mediates the activation of JNK by UVA radiation in human skin fibroblasts

FEBS Letters

Sies, Helmut¹  Klotz, Lars-Oliver¹  Briviba, Karlis¹ 

[1] Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Postfach 101007, D-40001 Düsseldorf, Germany

关键词: Singlet oxygen;    c-Jun–amino-terminal kinase;    Human skin fibroblast;    JNK;    c-Jun–N-terminal kinase;    UVA;    ultraviolet A radiation (320–400 nm);    RB;    Rose Bengal;   

DOI  :  10.1016/S0014-5793(97)00440-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ultraviolet A (UVA: 320–400 nm) radiation activates c-Jun–N-terminal kinase (JNK 2) in human skin fibroblasts. Exposure of cells to UVA (300 kJ/m²) led to a 5-fold induction of JNK-activity which was significantly increased in the presence of D₂O, an enhancer of the lifetime of singlet oxygen. Sodium azide, a quencher of singlet oxygen, abolished the activation of JNK. A hydroxyl radical scavenger, mannitol, had no effect. Furthermore, photochemically produced singlet oxygen (Rose Bengal plus white light) was found to induce JNK activity. This was enhanced by D₂O and inhibited by azide. Thus, singlet oxygen activates and mediates the UVA-induced activation of JNK.

【 授权许可】

Unknown