Regulation of the DNA binding of p53 by its interaction with protein kinase CK2

FEBS Letters

Schuster, Norbert¹  Montenarh, Mathias¹  Prowald, Alexandra¹ 

[1] Department of Medical Biochemistry, Building 44, University of the Saarland, D-66421 Homburg, Germany

关键词: Growth suppressor protein;    Protein kinase CK2;    DNA binding;    Protein–protein interaction;   

DOI  :  10.1016/S0014-5793(97)00399-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Some of the numerous functions of the growth suppressor protein p53 are regulated by its interaction with viral and cellular proteins. C-terminal sequences of p53 are implicated in binding to the regulatory β-subunit of protein kinase CK2. Using a p53-specific DNA binding element we found that the β-subunit of CK2 inhibited the DNA binding of p53 whereas the α-subunit had no influence. The CK2 holoenzyme consisting of two α- and two β-subunits led to a supershift in DNA binding of p53 similar to the p53-specific monoclonal antibody PAb421 as well as the C-terminus of p53. Thus, our results showed an individual role of the free β-subunit of CK2 on the DNA binding activity of p53.

【 授权许可】

Unknown