Production of formyl‐CoA during peroxisomal α‐oxidation of 3‐methyl‐branched fatty acids

FEBS Letters

Van Veldhoven, Paul P¹  Casteels, Minne¹  Croes, Kathleen¹  Mannaerts, Guy P¹ 

[1] Katholieke Universiteit Leuven, Afdeling Farmacologie, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium

关键词: Hydrolase;    Phytanic acid;    Refsum's disease;   

DOI  :  10.1016/S0014-5793(97)00343-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Oxidation of 3-methyl-substituted fatty acids was studied in purified rat liver peroxisomes. The experiments revealed that formyl-CoA is formed during the α-oxidation process. The amount of formyl-CoA found constituted 2–5% of the amount of formate formed. Under the conditions used, no activation of exogenously added formate occurred in purified peroxisomes, whereas 95.5% of added synthetic formyl-CoA was converted to formate. These data indicate that during α-oxidation first formyl-CoA is formed, which is then hydrolysed to formate.

【 授权许可】

Unknown