Formation of a 2‐methyl‐branched fatty aldehyde during peroxisomal α‐oxidation

FEBS Letters

Van Veldhoven, Paul P²  Herdewijn, Piet²  Asselberghs, Stanny¹  Casteels, Minne¹  Croes, Kathleen¹  Mannaerts, Guy P¹ 

[1] Katholieke Universiteit Leuven, Afdeling Farmacologie, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium;Rega Instituut, Medicinale Chemie, Minderbroederstraat, B-3000 Leuven, Belgium

关键词: Aldehyde;    α-Oxidation;    Formate;    Peroxisome;    Phytanic acid;    Refsum's disease;   

DOI  :  10.1016/S0014-5793(97)00856-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the final reaction of peroxisomal α-oxidation of 3-methyl-branched fatty acids a 2-hydroxy-3-methylacyl-CoA intermediate is cleaved to formyl-CoA and a hitherto unidentified product. The release of formyl-CoA suggests that the unidentified product may be a fatty aldehyde. When purified rat liver peroxisomes were incubated with 2-hydroxy-3-methylhexadecanoyl-CoA 2-methylpentadecanal was indeed formed. The production rates of formyl-CoA (measured as formate) and of the aldehyde were in the same range. While the production of formate remained unaltered in the presence of NAD⁺, the amount of 2-methylpentadecanal was decreased, which was accompanied by the formation of 2-methylpentadecanoic acid. These data indicate that (1) during α-oxidation the 2-hydroxy-3-methylacyl-CoA is cleaved to a 2-methyl-branched aldehyde and formyl-CoA and (2) liver peroxisomes are capable of converting this aldehyde to a 2-methyl-branched fatty acid.

【 授权许可】

Unknown