FEBS Letters | |
Formation of a 2‐methyl‐branched fatty aldehyde during peroxisomal α‐oxidation | |
Van Veldhoven, Paul P2  Herdewijn, Piet2  Asselberghs, Stanny1  Casteels, Minne1  Croes, Kathleen1  Mannaerts, Guy P1  | |
[1] Katholieke Universiteit Leuven, Afdeling Farmacologie, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium;Rega Instituut, Medicinale Chemie, Minderbroederstraat, B-3000 Leuven, Belgium | |
关键词: Aldehyde; α-Oxidation; Formate; Peroxisome; Phytanic acid; Refsum's disease; | |
DOI : 10.1016/S0014-5793(97)00856-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In the final reaction of peroxisomal α-oxidation of 3-methyl-branched fatty acids a 2-hydroxy-3-methylacyl-CoA intermediate is cleaved to formyl-CoA and a hitherto unidentified product. The release of formyl-CoA suggests that the unidentified product may be a fatty aldehyde. When purified rat liver peroxisomes were incubated with 2-hydroxy-3-methylhexadecanoyl-CoA 2-methylpentadecanal was indeed formed. The production rates of formyl-CoA (measured as formate) and of the aldehyde were in the same range. While the production of formate remained unaltered in the presence of NAD+, the amount of 2-methylpentadecanal was decreased, which was accompanied by the formation of 2-methylpentadecanoic acid. These data indicate that (1) during α-oxidation the 2-hydroxy-3-methylacyl-CoA is cleaved to a 2-methyl-branched aldehyde and formyl-CoA and (2) liver peroxisomes are capable of converting this aldehyde to a 2-methyl-branched fatty acid.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020304752ZK.pdf | 373KB | download |