Expression of recombinant human ceruloplasmin – an absolute requirement for splicing signals in the expression cassette

FEBS Letters

Choudhury, Natasha¹  Evans, Robert W¹  White, Kenneth N¹  Rafiq, Mineza¹  Joannou, Christopher L¹  Suen, Christine K.M¹ 

[1]Division of Biochemistry and Molecular Biology, United Medical and Dental Schools of Guy's and St Thomas's Hospitals, Guy's Hospital, London SE1 9RT, UK

关键词: Human ceruloplasmin;    Intron;    Expression cassette;    mRNA export;    In situ hybridization;    BHK;    baby hamster kidney;    Cp;    ceruloplasmin;    ISH;    in situ hybridisation;    DIG;    digoxigenin;    βGI;    β-globin intron;   

DOI  :  10.1016/S0014-5793(97)00325-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the successful expression of recombinant human ceruloplasmin which was made possible by inclusion of splicing signals in the expression vector. Ceruloplasmin cDNA expressed from the vector pNUT in baby hamster kidney cells gave protein yields of 0.03 mg/l which increased to 15 mg/l with splicing signals present. The defect in expression from the intronless cDNA is due to complete retention of ceruloplasmin mRNA in cell nuclei. The block to cytoplasmic export is alleviated by splicing signals, allowing full expression of the mRNA.

【 授权许可】

Unknown