Contribution of β‐lactamase production to the resistance of mycobacteria to β‐lactam antibiotics

FEBS Letters

Gicquel, Brigitte¹  Amicosante, Gianfranco³  Galleni, Moreno²  Reyrat, Jean-Marc¹  Pelicic, Vladimir¹  Frère, Jean-Marie²  Monnaie, Didier¹  Quinting, Birgit² 

[1] Unité de Génétique Mycobactérienne (CNRS URA 13000), Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France;Centre d'Ingénièrie des Proteines, Université de Liège, Institut de Chimie (B6), B-4000 Sart Tilman, Liège 1, Belgium;Department of Science and Biomedical Technology and Biometrics, Institute of Biological Chemistry and Molecular Biology, University of L'Aquila, L'Aquila, Italy

关键词: Mycobacterium fallax;    Mycolic acid;    β-Lactamase;    Diffusion;    Minimum inhibitory concentration;    MIC;    minimum inhibitory concentration;    PBP;    penicillin binding protein;   

DOI  :  10.1016/S0014-5793(97)00286-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mycobacterium fallax (M. fallax) is naturally sensitive to many β-lactam antibiotics (MIC<2 μg/ml) and devoid of β-lactamase activity. In this paper, we show that the production of the β-lactamase of Mycobacterium fortuitum by M. fallax significantly increased the MIC values for good substrates of the enzyme, whereas the potency of poor substrates or transient inactivators was not modified. The rates of diffusion of β-lactams through the mycolic acid layer were low, but for all studied compounds the half-equilibration times were such that they would only marginally affect the MIC values in the absence of β-lactamase production. These results emphasize the importance of enzymatic degradation as a major factor in the resistance of mycobacteria to penicillins.

【 授权许可】

Unknown