Cca3, the mRNA level of which transiently decreases before initiation of DNA synthesis in regenerating rat liver cells

FEBS Letters

Yuasa, Hirofumi²  Ichinose, Masutaro²  Ishibashi, Masahide¹  Tatematsu, Masae²  Hayashi, Yasuyuki¹ 

[1] Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa-ku, Nagoya 464, Japan;Laboratory of Pathology, Research Institute, Aichi Cancer Center, Chikusa-ku, Nagoya 464, Japan

关键词: Rat liver regeneration;    cca3 cDNA cloning;    Ankyrin repeat;    aa;    amino acid;    cca;    confluent 3Y1 cell-associated;    gas;    growth arrest-specific;    nt;    nucleotide;    ORF;    open reading frame;    PH;    partial hepatectomy;    RACE;    rapid amplification of the cDNA ends;    UTR;    untranslated DNA region;   

DOI  :  10.1016/S0014-5793(97)00258-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Kinetics of cca1, cca2, cca3 and rat gas1 mRNA levels were compared with those of DNA synthesis level in regenerating rat liver cells after partial hepatectomy. A transient decrease of cca3 mRNA level and an increase of rat gas1 mRNA level were observed before initiation of DNA synthesis, followed by a rapid decrease of rat gas1 mRNA level. By molecular cloning and nucleotide sequencing, cca3 cDNA was found to consist of 4514 nucleotides with a large open reading frame of 3027 nucleotides, encoding a protein of 1009 amino acids with three copies of an ankyrin repeat-like sequence.

【 授权许可】

Unknown