Cloning and expression of superoxide dismutase from Aquifex pyrophilus, a hyperthermophilic bacterium

FEBS Letters

Kim, Sung-Hou²  Yu, Yeon Gyu²  Han, Ye Sun²  Lim, Jae-Hwan²  Ahn, Byung-Yoon¹  Ryu, Jae-Ryeon²  Choi, In-Geol² 

[1] Department of Genetic Engineering, Korea University, Seoul, Korea;Structural Biology Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Korea

关键词: Hyperthermophile;    Superoxide dismutase;    Thermostability;    Aquifex;   

DOI  :  10.1016/S0014-5793(97)00262-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A superoxide dismutase (SOD) gene of Aquifex pyrophilus, a marine hyperthermophilic bacterium, was cloned, sequenced, expressed in Escherichia coli, and its gene product characterized. This is the first SOD from a hyperthermophilic bacterium that has been cloned. It is an iron-containing homo-oligomeric protein with a monomeric molecular mass of 24.2 kDa. The DNA-derived amino acid sequence is more similar to those of known Mn- and Fe-SODs from thermophilic archaea than of Cu, Zn-SODs. The metal binding residues found in all SOD sequences from different species are also conserved in A. pyrophilus SOD. The protein is biochemically active only as an oligomer and is resistant to thermal denaturation.

【 授权许可】

Unknown