Co‐localization of glucokinase with actin filaments

FEBS Letters

Ishihara, Hisamitsu²  Miwa, Ichitomo¹  Murata, Tomiyasu²  Oka, Yoshitomo³  Toyoda, Yukiyasu¹  Pekiner, Bilgehan¹  Pekiner, Can¹  Shibasaki, Yoshikazu²  Asano, Tomoichiro²  Katagiri, Hideki² 

[1] Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Tempaku-ku, Nagoya 468, Japan;Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;Third Department of Internal Medicine, Yamaguchi University School of Medicine, Kogushi, Ube, Yamaguchi 755, Japan

关键词: Liver glucokinase;    Islet glucokinase;    Actin filament;   

DOI  :  10.1016/S0014-5793(97)00253-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase was also co-localized with actin filaments in the cytoplasm of these transfected cells. Although co-localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co-localization with actin filaments, may have an important physiological role in metabolic regulation.

【 授权许可】

Unknown