A novel 40 000 Da Ca2+‐dependent actin modulator from bovine brain

FEBS Letters

Hurny, A.C.¹  Wnuk, W.¹ 

[1] Department of Biochemistry, University of Geneva, 30, quai E. Ansermet, 1211 Geneva 8, Switzerland

关键词: (Brain);    Actin filament;    Actin modulator;    Ca2+ dependence;    PMSF;    phenylmethylsulfonyl fluoride;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(86)81344-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monomeric protein of M _r 40000 that modulates the polymer state of actin has been isolated from bovine brain. When added either to preformed actin filaments or to monomeric actin, prior to polymerization, the modulator reduces the low-shear viscosity of F-actin provided that Ca²⁺ is present. The 40 kDa protein also inhibits the rate of actin polymerization. The inhibition is fully suppressed by removal of Ca²⁺ and restored by subsequent readdition of Ca²⁺, suggesting that the Ca²⁺-controlled interaction of actin with the 40 kDa modulator is freely reversible.

【 授权许可】

Unknown