A simple determination of steroidal alkaloid glycosides by thin‐layer chromatography immunostaining using monoclonal antibody against solamargine

FEBS Letters

Tanaka, Hiroyuki²  Shoyama, Yukihiro²  Tsuzaki, Chiyumi²  Putalun, Waraporn¹ 

[1]Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand

[2]Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan

关键词: Solasodine glycoside;    Solamargine-BSA conjugate;    Monoclonal antibody;    FAB-MS;    MALDI-MS;    TLC immunostaining;    TLC;    thin-layer chromatography;    PVDF;    polyvinylidene difluoride;    MALDI;    matrix-assisted laser desorption/ionization;    TOF;    time-of-flight;    BSA;    bovine serum albumin;    HSA;    human serum albumin;    TPBS;    phosphate buffer solution containing 0.05% Tween 20;    GPBS;    phosphate buffer solution containing 0.2% gelatin;    ELISA;    enzyme-linked immunosorbent assay;    MAb;    monoclonal antibody;    ABTS;    2;    2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt.;   

DOI  :  10.1016/S0014-5793(97)00106-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown