Mutagenesis of firefly luciferase shows that cysteine residues are not required for bioluminescence activity

FEBS Letters

Tsuji, Frederick I.¹  Ohmiya, Yoshihiro² 

[1] Marine Biology Research Division 0202, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093, USA;Department of Biochemistry, Faculty of Education, Shizuoka University, Ohya 836, Shizuoka 422, Japan

关键词: Photinus pyralis;    Oxygenase;    Active site;    Protein modification;    Amino acid sequence homology;    ATP;    adenosine 5′-triphosphate;    AMP;    adenosine 5′-monophosphate;    PPi;    pyrophosphate;    IPTG;    isopropyl-β-d-thiogalactoside;    Pp;    Photinus pyralis;    Hp;    Hotaria parvula;    Pm;    Pyrocoelia miyako;    PCR;    polymerase chain reaction;    TPCK;    N-tosyl-l-phenylalanine chloromethyl ketone;    TLCK;    N-tosyl-l-lysine chloromethyl ketone;    Val or V;    valine;    Ile or I;    isoleucine;    Lys or K;    lysine;    Cys or C;    cysteine;    Ser or S;    serine;   

DOI  :  10.1016/S0014-5793(97)00105-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

© 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown