Differential splicing of the growth hormone‐releasing hormone gene in rat placenta generates a novel pre‐proGHRH mRNA that encodes a different C‐terminal flanking peptide

FEBS Letters

Boronat, Albert¹  González-Crespo, Sergio¹  Pérez-Riba, Mercè¹ 

[1] Departament de Genètica, Institut de Recerca Oncològica, Autovia, Castelldefels km 2,7, 08907 Barcelona, Spain

关键词: Gestation;    Gene expression;    Hypothalamus;    Regulatory peptide;    GHRH;    growth hormone-releasing hormone;    nt;    nucleotide(s);    GCTP;    GHRH C-terminal peptide;   

DOI  :  10.1016/S0014-5793(97)00003-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and characterized a novel rat placental pre-proGHRH mRNA (pre-proGHRH-2 mRNA). This mRNA is generated by an alternative splicing process which results in the presence of an additional exon of 156 bp (designated exon 4.5) located between exons 4 and 5 of the previously reported hypothalamic and placental pre-proGHRH mRNA (pre-proGHRH-1 mRNA). Since the sequences encoding mature GHRH are included within exons 3 and 4, the processing of pre-proGHRH-2 would not affect the synthesis of mature GHRH but would generate a C-terminal peptide (designated GCTP-2) different from that previously reported in the hypothalamus and placenta (GCTP-1). The putative GCTP-2 has 64 amino acids, and the first 18 N-terminal residues are identical to those present in GCTP-1 (30 amino acids long). Pre-proGHRH-2 mRNA has not been detected in the hypothalamus.

【 授权许可】

Unknown