Mapping of a potent transcriptional repression region of the human homeodomain protein EVX1

FEBS Letters

Briata, Paola¹  Ilengo, Cristina¹  Corte, Giorgio²  Van DeWerken, Rinke¹ 

[1] Laboratory of Immunobiology I.S.T., Advanced Biotechnology Center, Largo R. Benzi, 10, 16132 Genova, Italy;Institute of Biochemistry, Genoa University Medical School, Genova, Italy

关键词: EVX1;    Gene transcription;    Repression domain;   

DOI  :  10.1016/S0014-5793(96)01512-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human homeodomain protein EVX1 is a transcriptional repressor in transfected mammalian cells and this function depends on a region carboxyl-terminal to the homeodomain. In this study, we transiently expressed several deletions of the EVX1 C-terminal region in mammalian cells and investigated their effect on the transcription of a reporter gene directed by different promoters. We show that the repressor activity maps to a region of 51 amino acids with a high abundance of alanine and proline residues. This region is able to transfer the repressor function to either the entire HOXC6 or CREB transcription factors, or to the GAL4 DNA binding domain.

【 授权许可】

Unknown