Liposome‐mediated DNA vaccination

FEBS Letters

de Souza, J.Brian²  Gregoriadis, Gregory¹  Saffie, Roghieh¹ 

[1] Centre for Drug Delivery Research, The School of Pharmacy, University of London, 29–39 Brunswick Square, London WC1N 1AX, UK;Department of Immunology, University College London Medical School, Arthur Stanley House, 40–50 Tottenham Street, London W1P 9PG, UK

关键词: Liposome;    Plasmid DNA;    DNA vaccination;   

DOI  :  10.1016/S0014-5793(96)01507-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Numerous reports have indicated that intramuscular injection of antigen-coding naked plasmid DNA can trigger humoral and cell-mediated protective immunity against infection. This follows DNA uptake by muscle fibres, leading to the expression and extracellular release of the antigen. Here it is shown for the first time that intramuscular immunization of mice with pRc/CMV HBS (encoding the S region of hepatitis B antigen; HBsAg) entrapped into positively charged (cationic) liposomes leads to greatly improved humoral and cell-mediated immunity. These cationic liposome-entrapped DNA vaccines generate titres of anti-HBsAg IgG₁ antibody isotype in excess of 100-fold higher and increased levels of both IFN-γ and IL-4 when compared with naked DNA or DNA complexed with preformed similar (cationic) liposomes. It is likely that immunization with liposome-entrapped plasmid DNA involves antigen-presenting cells locally or in the regional draining lymph nodes.

【 授权许可】

Unknown