C/EBPα is a major activator for the transcription of rat Cu/Zn superoxide dismutase gene in liver cell

FEBS Letters

Rho, Hyune Mo²  Yoo, Hae Yong²  Jung, Guhung¹  Chang, Mun Seog²  Kim, Young Ho² 

[1]Department of Biology Education, Seoul National University, Seoul 151-742, South Korea

[2]Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul 151-742, South Korea

关键词: Rat SOD1 gene;    C/EBPα;    Tissue distribution;    Transcription;    Transactivation;    SOD1;    Cu/Zn superoxide dismutase;    SOD1;    Cu/Zn superoxide dismutase gene;    C/EBP;    CCAAT/enhancer-binding protein;    HNF;    hepatocyte nuclear factor;    CAT;    chloramphenicol acetyl transferase;    MSA;    mobility shift assay;    nt;    nucleotide(s);    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(96)01487-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rat Cu/Zn superoxide dismutase (SOD1) is expressed in all tissues. Sequence analysis of the SOD1 promoter region showed that none of the cis-elements of hepatocyte-specific nuclear factors (HNF) were observed. The cis-element of C/EBPα in the proximal region of the SOD1 promoter and the high level of C/EBPα in the liver tissue led us to focus on the transcriptional regulation of the SOD1 gene by C/EBPα. Cotransfection assays with the plasmid expressing transcription factor C/EBPα showed that C/EBPα transactivated SOD1 gene by 27 fold. The marked transactivation and direct binding of C/EBPα to the SOD1 promoter were confirmed by deletion analyses and mobility shift assays. These results suggested that C/EBPα plays a major role in the tissue distribution of SOD1.

【 授权许可】

Unknown