Murine tissue inhibitor of metalloproteinases—4 (Timp—4): cDNA isolation and expression in adult mouse tissues

FEBS Letters

Taniguchi, Gary T.¹  Khokha, Rama³  Apte, Suneel S.²  Hawkes, Susan P.¹  Leco, Kevin J.⁴  Schultz, Gilbert A.⁴  Edwards, Dylan R.⁴ 

[1] Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0446, USA;Cleveland Clinic Foundation, Department of Biomedical Engineering, 9500 Euclid Avenue, Cleveland, OH 44195, USA;Ontario Cancer Institute, Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario M5G 2M5, Canada;Department of Medical Biochemistry, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada

关键词: Tissue inhibitor of metalloproteinases;    Extracellular matrix;    cDNA cloning;    Gene expression;    TIMP-4;    designates protein;    Timp-4 and TIMP4 are symbols for the mouse and human genes;    respectively;   

DOI  :  10.1016/S0014-5793(96)01474-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated cDNA clones corresponding to a new member of the murine tissue inhibitor of metalloproteinase (TIMP) family, designated Timp-4. The nucleotide sequence predicts a protein of 22 609 Da that contains the characteristic 12 cysteine TIMP signature. TIMP-4 is more closely related to TIMP-2 and TIMP-3 than to TIMP-1 (48%, 45% and 38% identity, respectively). Analysis of Timp-4 mRNA expression in adult mouse tissues indicated a 1.2 kb transcript in brain, heart, ovary and skeletal muscle. This pattern of expression distinguishes Timp-4 from other Timps, suggesting that the TIMP-4 protein may be an important tissue-specific regulator of extracellular matrix remodelling.

【 授权许可】

Unknown