Sucrose synthase genes in barley

FEBS Letters

de la Hoz, Pilar Sanchez¹  Vicente-Carbajosa, Jesus¹  Carbonero, Pilar¹  de Ilarduya, Oscar Martinez¹ 

[1] Catedra de Bioquímica y Biología Molecular, E.T.S. Ingenieros Agrónomes- UPM, E-28040 Madrid, Spain

关键词: Barley;    cDNA cloning;    Gene expression;    Northern blot;    Specific probe;    Sucrose synthase gene;   

DOI  :  10.1016/0014-5793(93)80087-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA of 2,708 bp encoding type 2 sucrose synthase (Ss2) from barley has been sequenced. Similarity of this cDNA with respect to that of type 1 (Ss1) is high in the coding region (75% identical positions), but low (41% identical residues) in the 3' non-coding region. Type-specific non cross-hybridizing probes for Northern blot analysis have been obtained from the 3' ends. The Ss1 type is highly expressed in developing endosperm and in roots and, at lower levels, in coleoptiles and aleurone. The Ss2 mRNA is abundant in endosperm, low in aleurone, and undetected in coleoptiles and roots.

【 授权许可】

Unknown