Homologous sucrose synthase genes in barley (Hordeum vulgare) are located in chromosomes 7H (syn. 1 and 2H Evidence for a gene translocation?

FEBS Letters

Vicente-Carbajosa, Jesús²  Mena, Montaña¹  Carbonero, Pilar²  de la Hoz, Pilar Sánchez² 

[1] Centro Nacional de Biotecnologia, Serrano, 115, 28006-Madrid, Spain;Cátedra de Bioquimica y Biologia Molecular, ETS Ingenieros Agrónomos- UPM, E-28040 Madrid, Spain

关键词: Barley;    cDNA cloning;    DNA mapping;    Sucrose synthase gene;    Wheat-barley addition line;   

DOI  :  10.1016/0014-5793(92)81143-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chromosomal location of the two types of sucrose synthase genes, Ss1 and Ss2, has been investigated in barley by Southern blot analysis of wheat-barley addition lines using non-cross-hybridizing-specific probes corresponding to the C-terminal regions of their respective CDNA clones (⋍250 bp). The Ss1 gene, whose cDNA of 2,667 bp has been entirely sequenced, is located in the β-arm of chromosome 7H (syn. 1), while that corresponding to the homologous Ss2 is in the short arm of 2H, suggesting the existence of a translocation event between these two chromosomes in cultivated barley after an initial gene duplication and divergent evolution.

【 授权许可】

Unknown