Aggregation of PDGF‐β receptors in human skin fibroblasts: characterization by image correlation spectroscopy (ICS)

FEBS Letters

Magnusson, Karl-Eric²  Petersen, Nils O³  Höddelius, Pia¹  Wiseman, Paul W³ 

[1] Department of Cell Biology, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden;Department of Medical Microbiology, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden;Department of Chemistry, the University of Western Ontario, London, Ontario N6A 5B7, Canada

关键词: Aggregation;    PDGF receptor;    PDGF;    Correlation spectroscopy;    Membrane domain;    CLSM;    confocal laser scanning microscopy;    EGF;    epidermal growth factor;    FRAP;    fluorescence recovery after photobleaching;    ICS;    image correlation spectroscopy;    PDGF-BB;    platelet-derived growth factor (BB);    PDGFR-β;    platelet-derived growth factor receptor-beta;    PKC;    protein kinase C;   

DOI  :  10.1016/S0014-5793(96)01429-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Receptor aggregation is believed to be an important, early step when growth factors such as PDGF stimulate proliferation and differentiation of cell populations. To investigate receptor aggregation, we utilized a novel biophysical technique, image correlation spectroscopy, to study the distribution and aggregation state of PDGF-β receptors on the surface of human dermal fibroblasts under various experimental conditions. It was found that the cell surface receptors were pre-clustered at 4°C and receptor aggregation increased for samples measured at 37°C. Treatment with PDGF-BB had no measurable effect on the receptor aggregation state. The results also indicate that additions of 10% serum or an inhibitor of tyrosine kinase activity, may disperse the receptors. The results of this study are consistent with organization of PDGF-β receptors in pre-existing membrane domains.

【 授权许可】

Unknown