Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes

FEBS Letters

Surdin-Kerjan, Yolande¹  Muldbjerg, Marianne²  Chérest, Hélène¹  Hansen, Jørgen² 

[1]Centre de Génétique Moléculaire du CNRS, 91198 Gif-sur-Yvette Cedex, France

[2]Carlsberg Research Laboratory, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark

关键词: Siroheme biosynthesis;    Yeast;    aa;    amino acid(s);    kb;    kilobasepair(s);    ORF;    open reading frame;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(96)01423-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Siroheme is a uroporphyrinogen III-derivative used by sulfite reductase as a prosthetic group. We investigated in Saccharomyces cerevisiae the possible involvement in siroheme biosynthesis of three genes, MET1, MET8 and MET20. The MET1 gene from S. cerevisiae was cloned and shown to be the same gene as MET20. Sequence similitudes as well as complementation studies indicate that Met1p and Met8p are both involved in siroheme biosynthesis. In addition, we show formally that S. cerevisiae does not need vitamin B₁₂ for growth.

【 授权许可】

Unknown