Monocyte chemoattractant protein‐2 can exert its effects through the MCP‐1 receptor (CC CKR2B)

FEBS Letters

Tanaka, Hiroko¹  Yamagami, Shinsuke¹  Endo, Noriaki¹ 

[1] Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical Research, 4-3-2 Asahigaoka, Hino, Tokyo 191, Japan

关键词: Monocyte chemoattractant protein;    Chemokine;    Chemokine receptor;    MCP;    monocyte chemoattractant protein;    MIP;    macrophage inflammatory protein;    RANTES;    regulated on activation;    normal T expressed and secreted;    EBNA;    EB virus nuclear antigen;    IC50;    concentration giving 50% inhibition;   

DOI  :  10.1016/S0014-5793(96)01411-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the activities of the monocyte chemoattractant proteins MCP-1, MCP-2 and MCP-3 on human embryonic kidney 293-EBNA cells transfected with the MCP-1 receptor (CC CKR2B). At 4 nM, MCP-2 induced a Ca²⁺ influx which was as potent as that with MCP-1 at 4 nM, although the increase by MCP-2 became saturated at higher concentrations. In addition, all three MCPs showed dose-dependent inhibition of adenylyl cyclase activity stimulated by forskolin (IC₅₀ values: 0.3 nM for MCP-1, 7 nM for MCP-2, and 1.5 nM for MCP-3). In conclusion, our data indicate that MCP-2 can exert its effects through the MCP-1 receptor, CC CKR2B.

【 授权许可】

Unknown