Substrate properties of C′‐methyl UTP derivatives in T7 RNA polymerase reactions. Evidence for N‐type NTP conformation

FEBS Letters

Padyukova, N.Sh¹  Chernyi, A.A¹  Lysov, Yu.P¹  Tunitskaya, V.L¹  Ermolinsky, B.S¹  Mikhailov, S.N¹  Rusakova, E.E¹  Kochetkov, S.N¹ 

[1] Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117984 Moscow, Russian Federation

关键词: T7 RNA polymerase;    C′-methyl analogue of UTP;    Transcription;    RNA chain terminator;    Conformational energy calculation;    Substrate conformation;   

DOI  :  10.1016/S0014-5793(96)01400-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5′ position) were shown to be weak substrates of T7 RNAP. 3′Me-UTP was neither substrate nor inhibitor of T7 RNAP while 2′Me-UTP was shown to terminate RNA chain synthesis. Conformational analysis of the analogues and parent nucleotide using the force-field method indicates that the allowed conformation of UTP during its incorporation into the growing RNA chain by T7 RNAP is limited to the χ angle range of 192–256° of N-type conformation.

【 授权许可】

Unknown