The MHC‐encoded TAP1/LMP2 bidirectional promoter is down‐regulated in highly oncogenic adenovirus type 12 transformed cells

FEBS Letters

Blair, G.Eric¹  Proffitt, James A¹ 

[1] Department of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK

关键词: Gene expression;    Nucleotide sequence;    Viral suppression of the MHC;    Ad;    adenovirus;    CAT;    chloramphenicol acetyltransferase;    LMP;    low molecular mass polypeptide;    MHC;    major histocompatibility complex;    TAP;    transporter associated with antigen processing;   

DOI  :  10.1016/S0014-5793(96)01375-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cells transformed by human adenovirus 12 (Ad12) exhibit extremely low surface levels of MHC class I molecules and contain reduced levels of class I heavy chain mRNAs. We report that levels of MHC-encoded TAP1 and LMP2 mRNAs are also down-regulated in Ad12-transformed rat cells, and that transcription of rat TAP1 and LMP2 transcripts is directed from a 564 bp intergenic region which is significantly less active in Ad12-transformed cells compared to those transformed with Ad5. Our results suggest that, in common with MHC class I gene expression, TAP1 and LMP2 gene expression is reduced mainly at the level of transcription in Ad12-transformed cells.

【 授权许可】

Unknown