Brain‐to‐blood active transport of β‐alanine across the blood–brain barrier

FEBS Letters

Sai, Yoshimichi¹  Komura, Junko¹  Tamai, Ikumi¹  Tsuji, Akira¹  Senmaru, Mizuho¹  Terasaki, Tetsuya¹ 

[1] Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Takara-machi 13-1, Kanazawa 920, Japan

关键词: Alanine;    β-;    Blood–brain barrier;    Antiluminal uptake;    Brain capillary endothelial cell;    Active transport;    Taurine;   

DOI  :  10.1016/S0014-5793(96)01366-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K _t is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by l-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

【 授权许可】

Unknown