Substrate activation behaviour of pyruvate decarboxylase from Pisum sativum cv. Miko

FEBS Letters

König, Stephan¹  Dietrich, Arndt¹ 

[1] Martin-Luther-Universität Halle-Wittenberg, Fachbereich Biochemie/Biotechnologie, Institut für Biochemie, Halle/Saale, Germany

关键词: Substrate activation;    Pyruvate decarboxylase;    SH-group;    Modification;    Thiamine diphosphate;    Pisum sativum cv. Miko;    ADH;    alcohol dehydrogenase;    PDC;    pyruvate decarboxylase;    HMB;    4-hydroxymercuri benzoate;    DTE;    1;    4 dithioerythritol;   

DOI  :  10.1016/S0014-5793(96)01326-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The substrate activation behaviour of pyruvate decarboxylase from germinating seeds of Pisum sativum is characterised kinetically via stopped-flow measurements and discussed with respect to other species. The involvement of SH-groups in this process is demonstrated by reference experiments with chemically modified enzyme.

【 授权许可】

Unknown