A CK2 site is reversibly phosphorylated in the photosystem II subunit CP29

FEBS Letters

Croce, Roberta²  Laureto, Patrizia Polverino-De¹  Testi, Maria Grazia²  Bassi, Roberto² 

[1] CRIBI, Centro Interdipartimentale Biotecnologie Innovative, Università di Padova, via Trieste 45, 35121, Padua, Italy;Universitá di Verona, Facoltá di Scienze MM.FF.NN., Biotecnologie Vegetali, Strada Le Gra-ie 37134, Verona, Italy

关键词: Cold stress;    Light-harvesting complex;    Chlorophyll;    Photosynthesis;    BBY;    PSII membrane preparation;    CK2;    casein kinase 2;    Chl;    chlorophyll;    CP;    chlorophyll-protein;    DM;    dodecyl-maltoside;    EDTA;    ethylenediaminetetraacetic acid;    ELFE;    electroendoosmotic electrophoresis;    HEPES;    N-2-(hydroxy-ethyl)piperazine-N′-2-ethane-sulfonic acid;    IEF;    isoelectrofocusing;    LHCII;    light-harvesting complex of PSII;    PAGE;    polyacrylamide gel electrophoresis;    PS;    photosystem;    rCP29;    recombinant CP29 reconstituted from the apoprotein derivatives overproduced in bacteria;    RC;    reaction centre;    SDS;    sodium dodecyl sulphate;    Tris;    2-amino-2-(hydroxymethyl)-1;    3-propanediol;   

DOI  :  10.1016/S0014-5793(96)01333-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein phosphorylation is a major mechanism in the regulation of protein function. In chloroplast thylakoids several photosystem II subunits, including the major antenna light-harvesting complex II and several core complex components, are reversibly phosphorylated depending on the redox state of the electron carriers. A previously unknown reversible phosphorylation event has recently been described on the CP29 subunit which leads to conformational changes and protection from cold stress (Bergantino, E., Dainese, P., Cerovic, Z. Sechi, S. and Bassi, R. (1995) J. Biol Chem. 270, 8474–8481). In this study, we have identified the phosphorylation site on the N-terminal, stroma-exposed domain, showing that it is located in a sequence not homologous to the other members of the Lhc family. The phosphorylated sequence is unique in chloroplast membranes since it meets the requirements for CK2 (casein kinase II) kinases. The possibility that this phosphorylation is involved in a signal transduction pathway is discussed.

【 授权许可】

Unknown