The chlorophyll a/b‐proteins of PS I and PS II are immunologically related

FEBS Letters

Evans, Paula K.¹  Anderson, Jan M.¹ 

[1] CSIRO, Division of Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia

关键词: Chlorophyll;    Light-harvesting complex;    Immunological cross-reactivity;    Immunological relationship;    Photosynthesis;    PS;    photosystem;    Chl;    chlorophyll;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(86)80485-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antisera were raised against three apoproteins of the light-harvesting chlorophyll a/b-protein complex of photosystem I (LHC I) from spinach. These antisera were found to cross-react with all four LHC I and both LHC II apoproteins on immunoblots. Affinity purification of the cross-reactive antibodies on either the ‘native’ LHC II complex or on the 28 kDa apoprotein of LHC II immobilized on nitrocellulose yielded antibodies which recognize the same apoproteins as the crude sera. Partial peptide mapping of LHC I and LHC II apoproteins with Staphylococcus aureus V8 protease and N-chlorosuccinimide showed different patterns for each polypeptide. These data demonstrate that the four polypeptides of LHC I are distinct from each other and from the two major polypeptides of LHC II but all six are immunologically related.

【 授权许可】

Unknown