Inhibition by penem of processing peptidases from cyanobacteria and chloroplast thylakoids

FEBS Letters

Barbrook, Adrian C.¹  Howe, Christopher J.¹  Packer, Jeremy C.L.¹ 

[1] Department of Biochemistry and Cambridge Centre for Molecular Recognition, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK

关键词: Thylakoid processing peptidase;    Signal peptidase;    Penem;    Phormidium laminosum;    Pisum sativum;   

DOI  :  10.1016/S0014-5793(96)01239-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins targeted to the thylakoid lumen of plants and cyanobacteria and the periplasmic space of cyanobacteria are synthesised with N-terminal presequences which are removed following translocation across the membrane. These presequences are thought to direct translocation of the preprotein by a sec-type pathway. Detergent extracts of cyanobacterial and chloroplast membranes contain enzymes which are capable of processing precursors to the mature size. We show that the processing of a range of precursors by both cyanobacterial and chloroplast enzymes is inhibited by the penem SB216357. This is the first report of an inhibitor of these enzymes and indicates that they are type 1 signal peptidases.

【 授权许可】

Unknown