Estimation of the functional size of Photosystem II

FEBS Letters

Nugent, J.H.A.¹  Atkinson, Y.E.¹ 

[1] Department of Botany and Microbiology, University College, Gower Street, London WC1E 6BT, England

关键词: Phormidium laminosum;    Photosystem II;    Photosynthesis;    Oxygen evolution;    Radiation inactivation;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    DMBQ;    2;    6-dimethyl-1;    4-benzoquinone;    ESR;    electron spin resonance;    NAD;    nicotinamide-adenine dinucleotide;   

DOI  :  10.1016/0014-5793(84)81375-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The radiation inactivation technique was used to measure the functional size of Photosystem II from Phormidium laminosum. Using frozen samples irradiated in liquid nitrogen, the technique was shown to give reproducible results. The mean functional size of PS II was found to be 125 kDa in a range from 99 kDa to 160 kDa. Comparing this result to those reported in other studies using SDS—PAGE suggests this functional size could be composed of one 52-kDa reaction centre polypeptide, one 33-kDa herbicide binding protein, and one 34-kDa polypeptide associated with oxygen evolution. Even the maximum limit of the estimate would only allow the further inclusion of approximately 40 kDa of polypeptide. The result suggests a smaller and simpler composition for PS II than indicated by SDS—PAGE.

【 授权许可】

Unknown