Fish testicular 11β‐hydroxylase : cDNA cloning and mRNA expression during spermatogenesis

FEBS Letters

Nonaka, Yasuki²  Kobayashi, Tohru¹  Nagahama, Yoshitaka¹  Ge, Wei¹  Okamoto, Mitsuhiro²  Jianga, Jian-Qiao¹  Kobayashi, Hiroko¹  Tanaka, Minoru¹ 

[1] Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444, Japan;Department of Molecular Physiological Chemistry, Osaka University Medical School, Osaka 565, Japan

关键词: Cytochrome P450(llβ);    cDNA cloning;    Spermatogenesis;    Japanese eel;   

DOI  :  10.1016/S0014-5793(96)01187-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated and characterized a cDNA encoding testicular 11β-hydroxylase, cytochrome P450(11β) from the Japanese eel (Anguilla japonica) testis. The cDNA contains an open reading frame that encodes a protein of 511 amino acids. The predicted amino acid sequence shares 38–48% homology with those of adrenal P450(11β) from mammals and frog. Transient expression in COS 1 cells confirmed that the protein encoded by this cDNA had P450(11β) activity. Northern blotting revealed a single 1.8 kb long transcript of P450(11β). This transcript was not found in immature eel testes prior to an injection with human chorionic gonadotropin (hCG), but it was present in eel testes after hCG injection.

【 授权许可】

Unknown