Carrier‐mediated transport controls hydroxyproline catabolism in heart mitochondria from spontaneously hypertensive rat

FEBS Letters

Minervini, Giuseppe M.²  Passarella, Salvatore³  Atlante, Anna²  Seccia, Teresa M.¹  Marra, Ersilia²  Vulpis, Vito¹  Pirrelli, Anna¹ 

[1] Dipartimento di Scienze Biomediche e Oncologia Umana, Università di Bari, Bari, Italy;Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, Bari, Italy;Dipartimento SAVA, Università del Molise, Campobasso, Italy

关键词: Hypertension;    Hydroxyproline;    Mitochondria;    Transport;    Collagen;    HYP;    hydroxyproline;    3-OH-P5C;    3-hydroxypyrroline-5-carboxylate;    4-OH-GLU;    4-hydroxyglutamate;   

DOI  :  10.1016/0014-5793(96)01114-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study we have investigated hydroxyproline transport in rat heart mitochondria and, in particular, in heart left ventricle mitochondria isolated from both spontaneously hypertensive and Wistar-Kyoto rats. Hydroxyproline uptake by mitochondria, where its catabolism takes place, occurs via a carrier-mediated process as demonstrated by the occurrence of both saturation kinetics and the inhibition shown by phenylsuccinate and the thiol reagent mersalyl. In any case, hydroxyproline transport was found to limit the rate of mitochondrial hydroxyproline catabolism. A significant change in V _max and K _m values was found in mitochondria from hypertensive/hypertrophied rats in which the K _m value decreases and the V _max value increases with respect to normotensive rats, thus accounting for the increase of hydroxyproline metabolism due to its increased concentration in a hypertrophic/hypertensive state.

【 授权许可】

Unknown