Existence of nuclear‐encoded 5S‐rRNA in bovine mitochondria

FEBS Letters

Ueda, Takuya³  Watanabe, Kimitsuna³  Koike, Tomohiro¹  Yoshionari, Shigeo³  Nishikawa, Kazuya¹  Miura, Kinichiro²  Yokogawa, Takashi¹ 

[1] Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 227, Japan;Institute for Biomolecular Science, Gakushuin University, Mejiro, Toshima-ku, Tokyo 171, Japan;Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: 5S-rRNA;    Transport;    Mitochondria;    Bovine;   

DOI  :  10.1016/0014-5793(94)80351-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A number of proteins functioning in mitochondria are synthesized in the cytoplasm and imported into the mitochondria via specific transport systems. In mammals, on the contrary, mitochondrial membranes have generally been considered to be impermeable to nucleic acids. However, here we show that an RNA with 120 nucleotides, the sequence of which is identical to that of the nuclear-encoded 5S RNA, exists in bovine mitochondria, although the mitochondrial genome encodes no 5S RNA gene. This RNA molecule was found to be retained in purified bovine mitochondria as well as in the mitoplasts, even after extensive treatment with an RNase, demonstrating that the 5S RNA is actually located inside the mitochondrial inner membrane. The 5S rRNA molecule was also shown to exist in mitochondria from rabbit and chicken.

【 授权许可】

Unknown