Nitric oxide stimulates stress‐activated protein kinases in glomerular endothelial and mesangial cells

FEBS Letters

Pfeilschifter, Josef¹  Huwiler, Andrea¹ 

[1] Department of Pharmacology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland

关键词: Nitric oxide;    Stress-activated protein kinase;    Mesangial cell;    Endothelial cell;    Apoptosis;   

DOI  :  10.1016/0014-5793(96)01070-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of rat glomerular mesangial cells and primary cultures of bovine glomerular endothelial cells to compounds releasing nitric oxide (NO), including MAHMANONOate, S-nitrosoglutathione, and spermine-NO, results in a time- and concentration-dependent activation of stress-activated protein kinases (SAPK) as measured by the phosphorylation of c-Jun in a solid phase kinase assay. Dibutyryl cGMP had no effect on SAPK activity. Pretreatment of the cells with the tyrosine kinase inhibitor genistein strongly attenuated NO-induced c-Jun phosphorylation. Furthermore, N-acetylcysteine markedly reduced the activation of SAPK in response to NO. These studies identify SAPK as a target for NO which may be critical for the NO-induced apoptosis of glomerular mesangial and endothelial cells.

【 授权许可】

Unknown