FEBS Letters | |
Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein‐3 | |
Clark-Lewis, Ian2  Sykes, Brian D1  Kim, Key-Sun1  Rajarathnam, Krishnakumar1  | |
[1] The Protein Engineering Network of Centres of Excellence (PENCE) and Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S2, Canada;The Biomedical Research Centre and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada | |
关键词: Monocyte chemotactic protein; NMR; Solution structure; Monomer-dimer; IL-8; interleukin-8; MCP; monocyte chemoattractant protein; MGSA; melanoma growth stimulatory activity; MIP-1β; macrophage inhibitory protein-1β; NAP-2; neutrophil activating peptide-2; PF-4; platelet factor-4; DQF-COSY; double quantum filtered correlation spectroscopy; NOE; nuclear Overhauser effect; NOESY; NOE spectroscopy; RANTES; regulated on activation normal T cell expressed; HPLC; high-performance liquid chromatography; RMS; root-mean-square; | |
DOI : 10.1016/0014-5793(96)01024-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (∼0.2–2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.
【 授权许可】
Unknown
【 预 览 】
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RO201912020303402ZK.pdf | 495KB | download |