Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein‐3

FEBS Letters

Clark-Lewis, Ian²  Sykes, Brian D¹  Kim, Key-Sun¹  Rajarathnam, Krishnakumar¹ 

[1] The Protein Engineering Network of Centres of Excellence (PENCE) and Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S2, Canada;The Biomedical Research Centre and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada

关键词: Monocyte chemotactic protein;    NMR;    Solution structure;    Monomer-dimer;    IL-8;    interleukin-8;    MCP;    monocyte chemoattractant protein;    MGSA;    melanoma growth stimulatory activity;    MIP-1β;    macrophage inhibitory protein-1β;    NAP-2;    neutrophil activating peptide-2;    PF-4;    platelet factor-4;    DQF-COSY;    double quantum filtered correlation spectroscopy;    NOE;    nuclear Overhauser effect;    NOESY;    NOE spectroscopy;    RANTES;    regulated on activation normal T cell expressed;    HPLC;    high-performance liquid chromatography;    RMS;    root-mean-square;   

DOI  :  10.1016/0014-5793(96)01024-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-2 show a monomer-dimer equilibrium in sedimentation equilibrium studies (∼0.2–2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.

【 授权许可】

Unknown