Galectin‐3 stimulates uptake of extracellular Ca2+ in human Jurkat T‐cells

FEBS Letters

Hughes, R.Colin¹  Dong, Sucai¹ 

[1] National Institute for Medical Research, Mill Hill, London NW7 1AA, UK

关键词: Jurkat T-cell;    Galectin-3;    Extracellular Ca2+;   

DOI  :  10.1016/0014-5793(96)01031-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Galectin-3, a mammalian galactoside-binding protein, is not expressed in the Jurkat T-lymphoblastoid cell line. However, Jurkat cells express surface glycoprotein receptors for galectin-3, one of which is shown to be the glycosylated heavy chain of CD98 (4F2 antigen), a T-cell activation marker. Addition of galectin-3 to Jurkat cells triggers a sustained influx of extracellular Ca²⁺ in a concentration dependent manner. The induced increase in cytosolic [Ca²⁺] _i is blocked by sugar hapten inhibitors of galectin-3. The galectin-3-induced effect is insensitive to voltage-gated Ca²⁺ channel antagonists such as prenylamine, nifedipine and diltiazem and to pertussis toxin but is inhibited by cholera toxin. The results suggest that galectin-3 released by accessory cells such as macrophages may bind in vivo to T-cell activation antigens and also participate in Ca²⁺ signalling.

【 授权许可】

Unknown