Hypo‐osmotic cell swelling activates the p38 MAP kinase signalling cascade

FEBS Letters

Gaestel, Matthias²  Engel, Katrin²  Edixhoven, Marcel J¹  de Jonge, Hugo R¹  Tilly, Ben C¹ 

[1] Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, The Netherlands;Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany

关键词: Regulatory volume decrease;    Chloride channel;    Reactivating kinase;    MAP kinase;    Heat shock protein;    Chloride channel;    Cytokine;   

DOI  :  10.1016/0014-5793(96)01028-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 is, similarly to that described for hyper-osmotic treatment, the result of an activated p38 MAP kinase cascade. The activation of MAPKAP kinase 2 proceeds with kinetics similar to that of one of the first physiological responses of hypo-osmotic treatment, the opening of compensatory Cl⁻ channels. However, inhibition of the p38 MAP kinase cascade does not block the osmo-sensitive anion efflux and, vice versa, activation of p38 MAP kinase by cytokines and anisomycin does not increase the efflux. These results indicate that the p38 MAP kinase cascade is not directly involved in Cl⁻ channel activation but instead may play a role in subsequent cellular repair processes.

【 授权许可】

Unknown