Isolation and characterization of a novel monomeric zinc‐ and heme‐containing protein from bovine brain

FEBS Letters

Gehrig, Peter M.¹  Vašák, Milan¹  Bogumil, Ralf¹  Faller, Peter¹  Hasler, Daniel W.¹  Fundel, Sibylle M.¹  Pountney, Dean L.¹ 

[1] Biochemisches Institut der Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland

关键词: Zinc protein;    Heme;    Brain tissue;    Electrospray mass spectrometry;    TFA;    trifluoroacetic acid;    MCD;    magnetic circular dichroism;    ESI MS;    electrospray ionization mass spectrometry;   

DOI  :  10.1016/0014-5793(96)00997-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An acidic zinc- and heme-containing protein was isolated from the soluble fraction of bovine brain and has been purified to homogeneity. The zinc-heme protein is a monomeric globular protein with a molecular mass of 31200 Da as determined by electrospray mass spectrometry. The protein was isolated with 0.90±0.05 zinc per protein and with substoichiometric amounts of heme. Amino acid sequences of four peptides (ca. 20% of the protein) were determined and the comparison of these sequences with those of protein and gene sequence databases revealed no significant correlation with any known protein. Thus, it is concluded that it is a novel protein of currently unknown biological function.

【 授权许可】

Unknown