Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides

FEBS Letters

Jégou, Sylvie¹  Lihrmann, Isabelle¹  Michael Conlon, J.²  Vaudry, Hubert¹  Alexandre, David¹  Anouar, Youssef¹ 

[1] European Institute for Peptide Research (IFRMP No. 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France;Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178, USA

关键词: Secretogranin II;    Chromogranin;    Secretoneurin;    Peptide precursor;    Frog;    CgA;    chromogranin A;    CgB;    chromogranin B;    PCR;    polymerase chain reaction;    5′ RACE;    rapid amplification of cDNA 5′ end;    SgII;    secretogranin II;    SN;    secretoneurin;   

DOI  :  10.1016/0014-5793(96)00976-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SgII in the brain of the frog Rana ridibunda, degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46–48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68–82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ∼4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII-derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.

【 授权许可】

Unknown