Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices

FEBS Letters

Picatoste, Fernando¹  Servitja, Joan-Marc¹  Claro, Enrique¹  Sarri, Elisabet¹ 

[1] Departament de Bioquímica i Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, Campus U.A.B., E-08193 Bellaterra, Spain

关键词: Phospholipase D;    Phosphatidylethanol;    Rat hippocampus;    PLD;    phospholipase D;    PtdEtOH;    phosphatidylethanol;    PtdCho;    phosphatidylcholine;    PtdEtn;    phosphatidylethanolamine;    PtdIns;    phosphatidylinositol;    PtdSer;    phosphatidylserine;    PtdOH;    phosphatidic acid;    DAG;    diacylglycerol;    TLC;    thin layer chromatography;   

DOI  :  10.1016/0014-5793(96)00906-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Noradrenaline- and ionomycin-stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [³²P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using ³H-fatty acids showed that two different classes of phosphatidylcholine, separated by two-dimensional TLC, one enriched with high incorporation of [³H]arachidonic acid (B) and the other with [³H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual ³²P-phospholipids extracted from [³²P]Pi-labeled hippocampal slices were incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [³²P]phosphatidylcholine, i.e. A and B, produced a different band of [³²P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.

【 授权许可】

Unknown